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2006•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Baicalin and Chlorogenic Acid in Yinhuang Capsule by HPLC

Yuguang Zheng

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Abstract

Objective To establish the HPLC assay for determination of chlorogenic acid and baicalin in Yinhuang capsule.MethodsThe chromatographic column of Hypersil ODS Agilent(4.6mm×250mm,5μm) was used.The mobile phase for analysis was consisted of methanol-0.1% phosphoric acid solution(gradient elution),the rate of flow was 1ml/min,and the detective wavelength was at 318nm.ResultsThe calibration curve of chlorogenic acid presented the favourable linear correlation over the range of 0.100 4~1.506 μg,and regression equation was Y=15.114 9+2 844.854 7X,r=0.999 9.The calibration curve of baicalin presented the favourable linear correlation over the range of 0.412 0~6.18 μg,and regression equation was Y=34.813 1+(2 190.058 7)X,r=0.999 9(n=5).ConclusionThe analytical method is convenient,accurate,stable,reliable,better reproducible,and it can be used for the quality control of Yinhuang Capsule.

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Objective To establish the HPLC assay for determination of chlorogenic acid and baicalin in Yinhuang capsule.MethodsThe chromatographic column of Hypersil ODS Agilent(4.6mm×250mm,5μm) was used.The mobile phase for analysis was consisted of methanol-0.1% phosphoric acid solution(gradient elution),the rate of flow was 1ml/min,and the detective wavelength was at 318nm.ResultsThe calibration curve of chlorogenic acid presented the favourable linear correlation over the range of 0.100 4~1.506 μg,and regression equation was Y=15.114 9+2 844.854 7X,r=0.999 9.The calibration curve of baicalin presented the favourable linear correlation over the range of 0.412 0~6.18 μg,and regression equation was Y=34.813 1+(2 190.058 7)X,r=0.999 9(n=5).ConclusionThe analytical method is convenient,accurate,stable,reliable,better reproducible,and it can be used for the quality control of Yinhuang Capsule.

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Available abstract

Objective To establish the HPLC assay for determination of chlorogenic acid and baicalin in Yinhuang capsule.MethodsThe chromatographic column of Hypersil ODS Agilent(4.6mm×250mm,5μm) was used.The mobile phase for analysis was consisted of methanol-0.1% phosphoric acid solution(gradient elution),the rate of flow was 1ml/min,and the detective wavelength was at 318nm.ResultsThe calibration curve of chlorogenic acid presented the favourable linear correlation over the range of 0.100 4~1.506 μg,and regression equation was Y=15.114 9+2 844.854 7X,r=0.999 9.The calibration curve of baicalin presented the favourable linear correlation over the range of 0.412 0~6.18 μg,and regression equation was Y=34.813 1+(2 190.058 7)X,r=0.999 9(n=5).ConclusionThe analytical method is convenient,accurate,stable,reliable,better reproducible,and it can be used for the quality control of Yinhuang Capsule.

Key concepts: Baicalin, Chlorogenic acid, Calibration curve, Chromatography, Phosphoric acid, Capsule, High-performance liquid chromatography, Chemistry

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