2011•Chinese Journal of Ethnomedicine and EthnopharmacyRequires access

Determination of chlorogenic acid and baical in Yinhuang Tablets by HPLC

LI Dong-mei

Abstract

Objective: To establish a HPLC method for determination of chlorogenic acid and baicalin in Yinhuang tablets.Method: In this method,the C18 column(4.6×150mm,5μm,shim-pack,Shimadzu)was used as the stationary phase,acetonitrile-0.1%phosphoric acid as gradient eluent,the flow rate was 1.0ml·min-1 and detection wavelength was at 318nm,the temperature of column was the room temperature.Result: The chlorogenic acid recovery of the added sample was 100.22% and RSD was 1.49%.The baicalin recovery of the added sample was 100.45% and RSD was 1.16%.Conclusion: This methods was simple,accurate and can be used for the quality control of YinHuang Tablets.

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Key concepts: Chromatography, Chlorogenic acid, Phosphoric acid, Baicalin, High-performance liquid chromatography, Chemistry, Organic chemistry