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2014•Traditional Chinese Drug Research and Clinical PharmacologyRequires access

Content Determination of Five Constitutes of Semen Cuscutae by HPLC

LI Huaigu

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Abstract

Objective To establish a method for the content determination of chlorogenic acid, hyperoside,isoquercitrin, astragalin and kaempferol in Semen Cuscutae by high performance liquid chromatography(HPLC).Methods HPLC was performed on Zorbax SB C18column,the acetonitrile-0.1 % phosphoric acid(gradient elution)was employed as the mobile phase,and detection wavelength was 327 nm for chlorogenic acid and 360 nm for hyperoside,isoquercitrin,astragalin and kaempferol. Column temperature was 30 ℃,and flow rate was 1.0mL·min-1. Results Baseline separation were obtained and linear relationship were good(r≥0.9998)for the five main constitutes. The average recoveries were 96.8 % ~101.0 % and RSD 3.0 %(n =6). Conclusion The establishedmethod is sensitive,reliable and available for the quality control of Semen Cuscutae

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Objective To establish a method for the content determination of chlorogenic acid, hyperoside,isoquercitrin, astragalin and kaempferol in Semen Cuscutae by high performance liquid chromatography(HPLC).Methods HPLC was performed on Zorbax SB C18column,the acetonitrile-0.1 % phosphoric acid(gradient elution)was employed as the mobile phase,and detection wavelength was 327 nm for chlorogenic acid and 360 nm for hyperoside,isoquercitrin,astragalin and kaempferol. Column temperature was 30 ℃,and flow rate was 1.0mL·min-1. Results Baseline separation were obtained and linear relationship were good(r≥0.9998)for the five main constitutes. The average recoveries were 96.8 % ~101.0 % and RSD 3.0 %(n =6). Conclusion The establishedmethod is sensitive,reliable and available for the quality control of Semen Cuscutae

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Available abstract

Objective To establish a method for the content determination of chlorogenic acid, hyperoside,isoquercitrin, astragalin and kaempferol in Semen Cuscutae by high performance liquid chromatography(HPLC).Methods HPLC was performed on Zorbax SB C18column,the acetonitrile-0.1 % phosphoric acid(gradient elution)was employed as the mobile phase,and detection wavelength was 327 nm for chlorogenic acid and 360 nm for hyperoside,isoquercitrin,astragalin and kaempferol. Column temperature was 30 ℃,and flow rate was 1.0mL·min-1. Results Baseline separation were obtained and linear relationship were good(r≥0.9998)for the five main constitutes. The average recoveries were 96.8 % ~101.0 % and RSD 3.0 %(n =6). Conclusion The establishedmethod is sensitive,reliable and available for the quality control of Semen Cuscutae

Key concepts: Astragalin, Hyperoside, Chromatography, Phosphoric acid, Chlorogenic acid, Chemistry, Kaempferol, High-performance liquid chromatography

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