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Study on Simultaneous Determination of Five Kinds of Flavonoids in Seed of Semen Cuscutae by HPLC

LV Jianb

Open publisher page 1 citations

Abstract

Objective: To determine the content of five kinds of flavonoids in the seed of semen cuscutae,including hyperoside,astragalin,quercetin,kaempferol and isorhamnetin. Methods: The HPLC method was used to determine the content of flavonoids in the seeds of semen cuscutae. The analysis was carried out on Agilent TC C18 column( 250 mm × 4. 6 mm,5 μm),with MeOH- 0. 4 % H3PO4as mobile phase,the flow rate of 1 mL·min- 1and wave length at 360 nm. The gradient elute time was 0→60min→80min,and the proportion of methanol was 25% →40% →60%. at45℃. Results: Chromatographic peak separation was in good condition,and five kinds of flavonoids had good linear relationship respectively within their own scope of concentration; the recovery was 98. 0% ~ 98. 0%,RSD was 1. 44% ~ 2. 63%. Conclusion: The established method is simple,accurate,and reproducible and can be used as the quality evaluation of semen cuscutae.

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Objective: To determine the content of five kinds of flavonoids in the seed of semen cuscutae,including hyperoside,astragalin,quercetin,kaempferol and isorhamnetin. Methods: The HPLC method was used to determine the content of flavonoids in the seeds of semen cuscutae. The analysis was carried out on Agilent TC C18 column( 250 mm × 4. 6 mm,5 μm),with MeOH- 0. 4 % H3PO4as mobile phase,the flow rate of 1 mL·min- 1and wave length at 360 nm. The gradient elute time was 0→60min→80min,and the proportion of methanol was 25% →40% →60%. at45℃. Results: Chromatographic peak separation was in good condition,and five kinds of flavonoids had good linear relationship respectively within their own scope of concentration; the recovery was 98. 0% ~ 98. 0%,RSD was 1. 44% ~ 2. 63%. Conclusion: The established method is simple,accurate,and reproducible and can be used as the quality evaluation of semen cuscutae.

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Available abstract

Objective: To determine the content of five kinds of flavonoids in the seed of semen cuscutae,including hyperoside,astragalin,quercetin,kaempferol and isorhamnetin. Methods: The HPLC method was used to determine the content of flavonoids in the seeds of semen cuscutae. The analysis was carried out on Agilent TC C18 column( 250 mm × 4. 6 mm,5 μm),with MeOH- 0. 4 % H3PO4as mobile phase,the flow rate of 1 mL·min- 1and wave length at 360 nm. The gradient elute time was 0→60min→80min,and the proportion of methanol was 25% →40% →60%. at45℃. Results: Chromatographic peak separation was in good condition,and five kinds of flavonoids had good linear relationship respectively within their own scope of concentration; the recovery was 98. 0% ~ 98. 0%,RSD was 1. 44% ~ 2. 63%. Conclusion: The established method is simple,accurate,and reproducible and can be used as the quality evaluation of semen cuscutae.

Key concepts: Chromatography, Semen, Hyperoside, Astragalin, Kaempferol, Chemistry, Isorhamnetin, Content determination

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