ScholarLens Back to search
2009•Kunming Yike Daxue xuebaoRequires access

Effect of Extract from Tripterygium Hypoglaucum on the Proliferation of Human Umbilical Vein Endothelial Cells

Zhuo Yu

Open publisher page 0 citations

Abstract

Objective To study the effect of THW-4,an extract from Trypterygium hypoglaucum on the proliferation of human umbilical vein endothelial cells (HUVEC).Methods HUVEC were cultured in vitro and different concentrations of THW-4 were added in experimental groups.Cell proliferation was evaluated by cell counting, MTT and and cell cycle detection.Results The inhibitory effects of THW-4 on proliferation of HUVEC displayed a dose dependent manner,with the halfinhibition concentration value (IC5)0 of 1.09 ng/mL at 24 h.Analysis of cell cycle indicated the THW-4 (0.5～10 ng/mL) mainly lead to the blockage of HUVEC in G0/G1 stage.Conclusion THW-4 has an inhibitory effect on the proliferation of HUVEC in vitro,and it has no protection on regeneration of functional endothelium after artery balloon.

About this research paper

What this paper is about

Objective To study the effect of THW-4,an extract from Trypterygium hypoglaucum on the proliferation of human umbilical vein endothelial cells (HUVEC).Methods HUVEC were cultured in vitro and different concentrations of THW-4 were added in experimental groups.Cell proliferation was evaluated by cell counting, MTT and and cell cycle detection.Results The inhibitory effects of THW-4 on proliferation of HUVEC displayed a dose dependent manner,with the halfinhibition concentration value (IC5)0 of 1.09 ng/mL at 24 h.Analysis of cell cycle indicated the THW-4 (0.5～10 ng/mL) mainly lead to the blockage of HUVEC in G0/G1 stage.Conclusion THW-4 has an inhibitory effect on the proliferation of HUVEC in vitro,and it has no protection on regeneration of functional endothelium after artery balloon.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To study the effect of THW-4,an extract from Trypterygium hypoglaucum on the proliferation of human umbilical vein endothelial cells (HUVEC).Methods HUVEC were cultured in vitro and different concentrations of THW-4 were added in experimental groups.Cell proliferation was evaluated by cell counting, MTT and and cell cycle detection.Results The inhibitory effects of THW-4 on proliferation of HUVEC displayed a dose dependent manner,with the halfinhibition concentration value (IC5)0 of 1.09 ng/mL at 24 h.Analysis of cell cycle indicated the THW-4 (0.5～10 ng/mL) mainly lead to the blockage of HUVEC in G0/G1 stage.Conclusion THW-4 has an inhibitory effect on the proliferation of HUVEC in vitro,and it has no protection on regeneration of functional endothelium after artery balloon.

Key concepts: Umbilical vein, Cell growth, Human umbilical vein endothelial cell, In vitro, Chemistry, MTT assay, Pharmacology, Medicine

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of Extract from Tripterygium Hypoglaucum on the Proliferation of Human Umbilical Vein Endothelial Cells — Research Paper | ScholarLens