Effect of Porphyromonasgingivalis Lipopolysaccharide on Proliferation and Apoptosis in Human Umbilical Vein Endothelial Cells in vitro

Objective: To study the effect of porphyromonasgingivalis lipopolysaccharide(P.g-LPS) on proliferation and apoptosis in human umbilical vein endothelial cells(HUVEC) in vitro. Methods: The HUVEC were cultured with different concentrations of P.g-LPS as P.g-LPS 0 pg/ml(Control group) and P.g-LPS 50pg/ml, 100pg/ml, 1ng/ml and 10ng/ml groups respectively. After 36 hours incubation, the cell proliferation viability and apoptosis were detected by MTT method and flow cytometry assay, the caspase-3 activation was examined by Western blot analysis. Results: Compared with Control group, the P.g-LPS treated HUVEC showed obviously decreased proliferation viability, P0.01. While with the increased P.g-LPS concentrations, the cell proliferation viability decreased accordingly, P0.01, and the cell apoptosis and caspase-3 activation were increased accordingly. Conclusion: P.g-LPS may inhibit endothelial cell proliferation viability, promote caspase-3 activation and therefore, inducing cell apoptosis in vitro.