Optimization of organic solvent method for extraction of carotenoids from Perilla frutescens meal

Organic solvent method was optimized here to extract carotenoids from Perilla frutescens meal for the comprehensive utilization of Perilla frutescens meal.Through the single factor experiment,the influence of the extraction solvent,the ratio of acetone to methanol,the extraction temperature,the extraction time and the ratio of liquid to perilla meal respectively on the yield of carotenoids were studied.Orthogonal test indicated under the temperature of 50 ℃ and 8 h of extraction the optimum ratios of acetone to methanol(V/V) and solid to liquid(W/V) were 2∶1 and 1∶50 respectively.Under these conditions,the yield of carotenoids from Perilla frutescens meal reached 4.0 mg/(100 g).