2013•Hubei nongye kexueRequires access
Study on Extraction Techniques of Protein from Perilla
Tian Hai-jua
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Abstract
Perilla(Perilla frutescens) protein was prepared by using defatted perilla leaf and stem as materiel through alkaliextraction and acid-precipitation method. The processing conditions of perilla protein were studied and optimized by single factor tests and orthogonal experiment. The results showed that the optimum extraction conditions were, pH for alkali-extraction,8.0; solid to liquid ratio, 1∶10(m / V,g∶mL); alkali-extraction temperature, 40 ℃; pH for acid-precipitation, 4.0. Under these conditions, the yield of perilla protein was 20.51%. If assisted with 100 W ultrasonic during the extracting process under optimum conditions, the yield of perilla protein was 22.41%.