Cloning,Identification and Sequence Analysis of the Bovine Interleukin-2 Gene

Based on the published nucleotide sequence of bovine interleukin-2,a pair of RT-PCR primers were designed and synthesized.Total bovine cell RNA,isolated from ConA-stimulated peripheral blood of bovine,was used as template to generate complementary DNA by reverse transcription.The 0.477 kb DNA fragment was amplified by polymerase chain reaction(PCR),and cloned into the pMD18-T vector.DNA sequencing confirmed the fragment was bovine interleukin-2 and the sequence was identical to the sequence of IL-2 publised in GenBank;Sequence analysis showed that the cloned sequence has very high similarity with that of ovine and Bubalus bubalis IL-2,over 95%;Comparative analysis of both amino acid sequence and antigenecity showed that the interleukin-2 of bovine has high similarity to the one of ovine and Bubalus bubalis.