Cloning and Sequence Analysis of Bovine Interferon-gamma Gene

Based on the published nucleotide sequence of bovine interferon-gamma(BovIFN-γ)gene,a pair of RT-PCR primers were designed and synthesized.The mature peptide fragments of γ-interferon gene was amplified using reverse transcription polymerase chain reaction(RT-PCR)from total RNA of bovine peripheral blood lymphocytes,and then carried out cloning,sequencing and sequence analysis.The results showed that the mature peptide of BovIFN-γ gene can be successfully amplified by primers designed.Additionally,the homology between the amplified gene fragment sequence and the relevant sequence in GenBank can reach up to 99.77%。