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2009•Zhongguo yaolixue tongbaoRequires access

Cloning and sequence analysis of calmodulin 1 gene in guinea-pig

Liying Hao

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Abstract

Aim To clone and sequence the gene of calmodulin 1(CaM1) in guinea-pig(Cavia porcellus);to analyze the bio-information of the cloned gene.Methods Total RNA was extracted from heart tissue of guinea-pig.A DNA fragment of 447 bp from the gene code of CaM1 was amplified by RT-PCR method.Recombinant plasmid was constructed by inserting cDNA into cloning vector before DNA sequencing and sequence analyzing.Results The cloned CaM1 gene encoded 149 amino acid residues.The homology with other mammal's relative CaM1 genes was more than 90%.The restriction enzyme positions of this sequence were confirmed.Conclusion The cloning of CaM1 gene in guinea-pig makes it a fundament for CaM expression and function research.

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What this paper is about

Aim To clone and sequence the gene of calmodulin 1(CaM1) in guinea-pig(Cavia porcellus);to analyze the bio-information of the cloned gene.Methods Total RNA was extracted from heart tissue of guinea-pig.A DNA fragment of 447 bp from the gene code of CaM1 was amplified by RT-PCR method.Recombinant plasmid was constructed by inserting cDNA into cloning vector before DNA sequencing and sequence analyzing.Results The cloned CaM1 gene encoded 149 amino acid residues.The homology with other mammal's relative CaM1 genes was more than 90%.The restriction enzyme positions of this sequence were confirmed.Conclusion The cloning of CaM1 gene in guinea-pig makes it a fundament for CaM expression and function research.

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Available abstract

Aim To clone and sequence the gene of calmodulin 1(CaM1) in guinea-pig(Cavia porcellus);to analyze the bio-information of the cloned gene.Methods Total RNA was extracted from heart tissue of guinea-pig.A DNA fragment of 447 bp from the gene code of CaM1 was amplified by RT-PCR method.Recombinant plasmid was constructed by inserting cDNA into cloning vector before DNA sequencing and sequence analyzing.Results The cloned CaM1 gene encoded 149 amino acid residues.The homology with other mammal's relative CaM1 genes was more than 90%.The restriction enzyme positions of this sequence were confirmed.Conclusion The cloning of CaM1 gene in guinea-pig makes it a fundament for CaM expression and function research.

Key concepts: Biology, Gene, Molecular biology, Cavia, Molecular cloning, Cloning (programming), Complementary DNA, Sequence analysis

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