The Effect of Puerarin on Serum Interleukin-1β and Tumor Necrosis Factor-alpha of Rabbits with Hepatic Ischemia-reperfusion Injury

Objective:To determine the inhibitory effect of puerarin on the level of serum interleukin-1β(IL-1β) and tumor necrosis factor-alpha(TNF-α) following hepatic ischemia and reperfusion injury(HIRI) in rabbits.Methods:30 rabbits which were randomly divided into five groups: control group(group C),ischemia-reperfusion group(group I-R),Nimodipine group(group Nim),PurI group(group PurI) and PurⅡgroup(group PurⅡ).Blood samples were obtained through vein to measure the serum concentration of IL-1βand TNF-α as well as alanine aminotransferase(ALT) activity in 20mins before ischemia,ischemia 45min and 45min after reperfusion.The ultrastructure of the hepatic tissue was observed.Results:The levels of IL-1βand TNF-α and the activity of ALT in serum at different time points in group I-R were higher significantly than that of the group C(P0.05 and P0.01).The abnormal morphological changes of hepatocytes under electric microscope were more obvious in the group I-R than that in the group C.In the group PurI,PurⅡand Nim the levels of IL-1βand TNF-α and the activity of ALT in serum at different time points were remarkably lower than that of the group I-R(P0.05 and P0.01),the abnormal morphyological changes of hepatocytes were all alleviated markedly after treatment of Pur and Nim.Conclusion:Puerarin could effectively decrease the levels of IL-1βand TNF-α and protect the liver from ischemia and reperfusion injury.