Effects of pirinixic acid on serum tumor necrosis factor-α and interleukin-1β in rats with renal ischemia-reperfusion injury

Objective To investigate the effects of pirinixic acid (PA) on serum tumor necrosis factor-α (TNF-α) and interleukin-lβ (IL-1β) in rats with renal ischemia-reperfusion (I/R) injury.Methods Fifty-four male SD rats were equally randomized into 3 groups:sham operation group (S group),I/R group and PA 5 mg/kg + I/R group (PA group).The renal I/R injury model in rats was established by clamping blood supply of the kidney with vascular clamps for 60 min.Rats in S group were subjected to the same surgical procedures without clamping blood vessels.Rats in PA group were intraperitoneally administrated with the dose of PA 5 mg/kg 30 min before ischemia.In S group and I/R group,rats were given the same volume of saline at the same time.Blood samples were taken from abdominal aorta 4 h after reperfusion to measure the serum levels of TNF-α and IL-1β.Results Four h after renal I/R,the serum levels of TNF-α and IL-1β were (11.65 ± 1.15) ng/L and (230.80 ±31.82) ng/L in I/R group,and (7.83 ± 1.27) ng/L and (125.74 ± 18.03) ng/L in PA group.Group PA had lower serum levels of TNF-α and IL-1β than I/R group (P ＜ 0.05).Conclusion PA preconditioning could reduce the serum levels of TNF-α and IL-1β in rats with renal I/R injury. Key words: Pirinixic acid; Renal ischemia; Reperfusion injure; Tumor necrosis factor-α; Interleukin-1β