2009•Lishizhen Medicine and Materia Medica ResearchRequires access
Influence of Tongguan Capsule on the Expression of Vascular Endothelial Growth Factor in Tunica Intima in Rats after Balloon Injury
Cheng Yang
Open publisher page 0 citations
Abstract
Objective To observe the effects of Tongguan capsule on the expression of vascular endothelial growth factor and intimal hyperplasy in Tunica intima in rats after balloon injury.Methods60 male Sprague-Dawley rats whose left common carotid arteries were injured were randomly divided into 3 groups(n=18) :Group l(10mg·kg-1·d-1 of PS was given after injury),group 2(2mg·kg-1·d-1 of Simvastatin was given after injury)and group 3(600mg·kg-1·d-1 of Tongguan capsule was given after injury).18 rats from each group were euthanized by exsanguination in week 4,week 8 and week 12 after injury.The injured left common carotid arteries were carefully removed for HE and elastic fibers staining.Intima area,media area and IA/MA were performed in IPP6.0.which is an automatic computerized imaging analysis system.To observe the reparation after injury of blood vessel endothelium through detection of VEGF with immunohistochemistry.ResultsIn comparison with Group l,the expression of VEGF in tunica intima in rats after balloon injury in Group 3 increased obviously(P0.01).The changes of the expression of VEGF of Group 2 were similar to Group 3.The expression of VEGF of Group 3 increased obviously in week 12(P0.05).4 weeks after the operation,morphological analysis demonstrated that there were marked differences in the area of neointima proliferation among all the groups.ConclusionTongguan capsule can improve the expression of VEGF in tunica intima in rats after balloon injury and inhibit intimal hyperplasia of injured vascular.