Study on the Optimization of Liquid Culture Medium in Flammuling velutipes Cart.Sing

The aim of the study was to optimize the liquid culture medium in Flammuling velutipes Cart.Sing.In orthogonal experiment 4 major nutrient factor selected was carbon source,nitrogen source,inorganic salt and vitamin to study the effects of different carbon sources and nitrogen sources,salts and vitamins and their different proportion on the mycelial growth and determine the optimum culture medium for the mycelial growth in F.Velutipes The optimum carbon source for liquid culture of F.velutipes was soluble starch,the optimum nitrogen source was soybean powder,the optimum inorganic salt was KCl,and the optimum vitamin was VB2.The optimum formula of culture medium was as follows: 3% soluble starch,4% soybean flour,0.15%KCl,7.5 mg/100 ml VB2.The study provided a theoretical basis for the industrialization production of F.velutipes.