The Expression of Interluki-β protein and mRna after Cerebral Ishcemia in Rats

Abstract:To investigate the expression of Interleukin β (IL β) mRNA in cerebral ischemia rat, and identify the cellular source of IL 1β. Methods: Using in stiu hybridization technique to explore the expression of IL 1β mRNA in ischemic cortex of rats. To identify cellular source of IL 1β mRNA and protein, hybridization and double immunofluorescence labeling were used. Results: ①The normal and sham rats showed a weak expression of IL 1β mRNA in cortex (70.3±7.3,80.4±5.5).The positive cells of IL 1β mRNA were increased significantly after 2h, 6h, 12h reperfusion (120.2±13.5; 112.6±8.8; 106.6±9.0), and sustained to 24 h after reperfusion (84.6±7.8). ②Most of neurons expressed IL 1β mRNA, IL 1β mRNA also expressd in astrocytes and microglia. IL 1β protein coexpressed with astrocytes and microglia. We didn't detect the coexpression of IL 1β protein and neurons. Conclusion: It indicated that the expression of IL 1β mRNA was increased after cerebral ischemia, IL 1β may play an important role in pathogenesis of ischemic injury. The main source of IL 1β may be astrocytes and microglia.\;