Preparation of polysaccharides from Flammulina velutipes by microwave-enzyme synergistic extraction

Microwave-enzyme synergistic method was used to extract the polysaccharides from Flammulina velutipes,and the effects of extracting conditions on the yield of Flammulina velutipes polysaccharides was studied through the single factors and orthogonal test.Results showed that microwave-enzyme synergistic method could increase the yield of Flammulina velutipes polysaccharides effectively.The optimal extraction conditions were obtained as follows: enzymolysis temperature 45 ℃,enzymolysis time 1.5 h,pH 4.0,concentration of pectinas 0.4%(m∶m),microwave power 600 W,ratio of solid-liquid 1∶20(m∶V),extraction time 90 s,and times extraction 3.Under the optimal conditions,the yield of Flammulina velutipes polysaccharides by using microwave-enzymatic synergistic method was 21.76%.