Comparison and performance index evaluation between ABI 7700 and 7500 fluorescent quantitation instrument for polymerase chain reaction

Objective To evaluate and compare main performance index of ABI 7700 and 7500 fluorescent quantitation instruments for polymerase chain reaction made in USA.Methods Based on the evaluation criterion established by NCCLS,we estimated the indexes including the precision,accuracy,linearity,comparability,sensitivity and so on between the two instruments by quantitating HBV-DNA.Results Both ABI 7700 and ABI 7500 had good precision,with the between-group and inner-group CV of ABI 7700 being 3.67%~5.58% and 4.46%~6.53%,respectively;these two variables for ABI 7500 were 4.09%~5.61% and 4.73%~6.96%,respectively.Accuracy test found that the relative deviation of ABI 7700 and ABI 7500 was 0.93% and 2.97%,respectively when compared to standard quality control reagent.The linearity of two machines was 0.999 and 0.995,respectively.As for the sensitivity,the smallest identical concentration of HBV-DNA was 1000IU/ml.in addition,the coefficient correlation of two machines was 0.994,and linear regression equation was y=1.0694x-0.1372.Conclusion According to the detections and statistical analysis,we have confirmed that the two instruments' various indexes are stable and reliable and hence we regard them as perfect instruments for PCR.