Evaluation on methods for quantitative detection of procalcitonin

Objective To evaluate the analytical performance of two methods of enzyme-linked immunoassay and electrochemiluminescene immunoassay for quantitatively detecting procalcitonin(PCT)and to explore the correlation and consistency of their test results in order to provide the basis for a reasonable choice of the PCT detection system.Methods The enzyme-linked immunoassay adopted the mini VIDAS automatic fluorescence immunoassay analyzer and the electrochemiluminescene immunoassay adopted the Cobas E411 electrochemiluminescene immunoassay analyzer.The corresponding matching low and high values of quality control materials were detected for calculating the intra-day precision and the inter-day precision;the non-same batch number of fixed value standard material in the corresponding reagent was determined for observing the bias degrees;the correlation and consistency of the detection results were analyzed and compared between the two kinds of method.Results The intra-day precisions of PCT in low and high values of quality control materials measured by the mini VIDAS fluorescence immunoassay analyzer were2.02% and 1.83%,the inter-day precisions were 3.59%,and 3.09%,the biases were 2.98% and-1.97%;the intra-day precisions of PCT measured by the Cobas E411 ECL analyzer were 1.30% and 0.87%,the inter-day precisions were 3.33% and 3.06%,the biases were 5.26%and 0.90%.The detection results of PCT by the two methods had statistically significant differences(P0.01),but there was good correlation(r=0.993)and good consistency(Kappa=0.76).Conclusion The analytical performance of two kinds of quantitative PCT detection method could meet the requirements of the laboratory with good correlation and good consistency.Laboratory could select proper detection system according to the actual situation.