HPLC-DAD-ELSD Simultaneous Determination of Four Active Components in Semen Ziziphi Spinosae(Suanzaoren) Dispersible Tablet

Objective:To establish an HPLC-DAD-ELSD method for the simultaneous determination of spinosin,jujuboside A,jujuboside B and betulinic acid in Semen Ziziphi Spinosa dispersible tablet.Method: The chromatographic separation was performed on a Venusil XBP reversed phase C18(4.6 mm×250 mm,5 μm) analytical column by a gradient elution program with acetonitrile-water(containing 0.1% acetic acid) at a flow rate of 1.0 mL·min-1.The column temperature was at 25 ℃ and the optimum detection wavelength of DAD was set at 335 nm,the evaporator tube temperature of ELSD was set at 100 ℃ with the nebulizing gas flow rate of 2.9 L ·-1.Result: The linear range of spinosin,jujuboside A,jujuboside B and betulinic acid were 3.36-32.36(r=0.999 2),1.12-11.24(r=0.999 6),1.36-13.56(r=0.999 7) 5.04-50.45 μg·g-1(r=0.999 3),respectively.The average recoveries were 99.23%(RSD 1.51%),101.25 %(RSD 1.49%),98.63%(RSD 2.57%),102.09%(RSD 0.72%),respectively.Conclusion: The method is simple and accurate,it can be used for the quality control of Semen Ziziphi Spinosae dispersible tablet.