Oxidative Stress Mechanism of Manganese-treated PC12 Cell Line

Objective: To investigate the apoptosis effect of Mn on the PC 12 cell line by detecting the cell morphology and biochemical changes.Methods: PC12 cells in logarithm period incubated in medim with 200,400,600,800μmol/L manganese(MnCl2) for 1day,2 days,3 days,4days respectively;The cell viability was detected by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrasoliumBromide];Morphological changes of PC12 cells was investigated by transmisssion electron microscope;Agarose gel electrophoresis was used to detect the genomic DNA of Mn-treated PC12 transmisssion electron microscope as well as biochemical hallmark of DNA fragments.Results: The results of MTT revealed that manganese of different Concentrations(MnCl2200,400,600,800μmol/L) could suppress the proliferation of PC12 cells in dose and time-dependent manner.The cell inhibited ratio at the fourth day in 600μmol/L MnCl2 culture medium approached 50% or more.In the same condition apoptosis was observed in cells.Conclusion: Mn has generated apoptosis which induced proliferation arrested of PC12 cells.