Relationship between expressions of P53,MDM2 proteins and manganese induced apoptosis in PC12 cells

Objective To study the relationship between apoptosis of PC12 cells induced by manganese and the levels of the expression of P53,MDM2 proteins.Methods PC12 cells in logarithm period were used as the model of dopaminergic neuron cells and incubated in culture media with 200,400,800 μmol/L manganese chloride(MnCl2) for 24,36,48 hours,respectively.MTT colorimetry test was performed to exam cell growth and livingness of PC12 cell exposed to various dose of manganese.Manganese induced apoptosis of PC12 cells was observed by flow cytometry(FCM)and terminal deoxynucleotidyl transferase-meidated dUTP nick end labeling(TUNEL)and immunohistochemistry was used to measure the expression of P53 and MDM2 proteins.Results Manganese suppress the proliferation of PC12 cells in dose and time-dependent manner and induce apoptosis of PC12 cells in dose-dependent manner.Manganese boosted the expression of P53 proteins and inhibited the expression of MDM2 protein in dose-dependent manner.Manganese induced apoptosis in PC12 cells was positively correlative to the expression of P53 and negatively correlative to the expression of MDM2.Conclusion Apoptosis of PC12 cells can be induced by manganese by enhancing the expression of P53 protein and prohibiting the expession of MDM2 protein.