The Experimental Studies of Apoptosis Induced by MPP~+ in PC12 Cells

By the techniques cell cultivation outside body, MTT assay, Fluorescence Staining, Transmission Electron Microscopy, and Flow Cytometry, we studied the effects of MPP~+ on proliferation and apoptosis in PC12 cells to provide experimental model for the study of neuroprotective effect. Result indicates that MPP~+ can induce apoptosis in PC12 cells, which showed the dependence on time and concentration. When PC12 cells were treated with 0.20 mM MPP~+ for 48 hours, cell viability was reduced to about 60% as determined by MTT assay, cell nuclear was agglomerated and ruptured, series changes was occurred in ultrastructure. All above results showed distinctive apoptosis characters.Annexin V-FITC apoptosis double-stained kit with Flow Cytometry shows that the visible cells were about 20.2%, late apoptosis/necrosis cells were about 51% and early apoptotic cells were about 6.75% It suggests that MPP~+ can induce apoptosis in PC12 cells, which is a valuable experimental model for the study of neuroprotective effect.