2002•Unpublished venueRequires access

Experimental Study of Baoshen Tablet on Fibroblasts of Rat Kidney

Xiao Zhu

Abstract

Objective:To study the effect of Baoshen tablet (BST) on fibroblasts from rat kidneyn in vitro. Methods:Fibroblasts for culture separated from SD rat kidney, added the rats serum with different concentration of BST to fibroblasts. The proliferation and apoptosis of fibroblasts were detected by MTT assay and flow cytometry respectively. Results:BST inhibited the proliferation of rat fibroblasts significantly compared with control( P0.05) and could induce cell apoptosis. Conclusion:The mechanism of BST postponing CRF may be in connection with the proliferation inhibition and apoptosis inducement of fibroblasts.

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Key concepts: Apoptosis, Flow cytometry, Fibroblast, In vitro, MTT assay, Cell growth, Kidney, Chemistry