Fluvastatin inhibiting proliferation of rat lung fibroblasts

Objective:To observe the inhibitory effect of fluvastatin (Flu) on the proliferation of the rat lung fibroblasts cultured in vitro . Methods: Normal rat lung derived fibroblasts were cultured in media containing Flu. The influences of Flu on the growth curve of the fibroblasts were observed by cell count and MTT colorimetry. The inhibition effect of Flu serial dilutions on the fibroblasts proliferation was investigated. Influence of Flu on division index of the fibroblasts was analyzed by direct cell count. Chemical colorimetry was used to detect the hydroxyproline in the media. Results: Flu inhibited the proliferation of the normal rat lung fibroblasts in the manner of dose dependence( P 0.01). Flu (10 -4 mol/L) completely inhibited proliferation of the fibroblasts. Division index and collagen production of cultured fibroblasts also decreased obviously under the action of Flu( P 0.01). Conclusion: Flu can inhibit the proliferation of the normal rat lung fibroblasts in vitro , and it might be an effective medicine for pulmonary fibrosis.