Separation and purification of total flavones from rape bee-pollen by macroporous resin
Yourui Suo
Abstract
Yourui Suo
Abstract
Purified the flavonoids from rape bee-pollen by 7 kinds of macroporous resins.AB-8 resin had much higher adsorption capacity and desorption rate than other resins.Then dynamic adsorption and desorption on the selected AB-8 resin were carried out,and the optimum parameters for separation were as follows:concentration 0.91mg/mL,pH 5,flow rate 2.5mL/min,the optimal eluant 50% ethanol with 3BV at the flow rate of 1mL/min.
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Purified the flavonoids from rape bee-pollen by 7 kinds of macroporous resins.AB-8 resin had much higher adsorption capacity and desorption rate than other resins.Then dynamic adsorption and desorption on the selected AB-8 resin were carried out,and the optimum parameters for separation were as follows:concentration 0.91mg/mL,pH 5,flow rate 2.5mL/min,the optimal eluant 50% ethanol with 3BV at the flow rate of 1mL/min.
Key concepts: Flavones, Adsorption, Desorption, Chromatography, Chemistry, Bee pollen, Elution, Pollen