2007Zhongnan Linye Keji Daxue xuebaoRequires access

Screening of Xylanase Producing Strain and Study of Its Characteristics

Hu Zhou

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Abstract

By enriching culture,hydrolysis ring,9 xylanase producing strains were screened from the soil on which corncobs piled for a long time.A high-yield xylanase producing strain was selected,whose characteristics were studied and with which enzymes were produced by fermentation.Results show that xylanase enzyme activity was high;it was 13.68 U/mL when temperature was at 28~30℃,original pH value about 5.0,turning speed 150 r/min,and time 48 h.Xylanase produced was heat1-resisting,its enzyme activity reserving 60.82 after 120 mins undertemperature 60℃.Its optimal temperature and pH value were 53℃ and pH 5.3 respectively.

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By enriching culture,hydrolysis ring,9 xylanase producing strains were screened from the soil on which corncobs piled for a long time.A high-yield xylanase producing strain was selected,whose characteristics were studied and with which enzymes were produced by fermentation.Results show that xylanase enzyme activity was high;it was 13.68 U/mL when temperature was at 28~30℃,original pH value about 5.0,turning speed 150 r/min,and time 48 h.Xylanase produced was heat1-resisting,its enzyme activity reserving 60.82 after 120 mins undertemperature 60℃.Its optimal temperature and pH value were 53℃ and pH 5.3 respectively.

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Available abstract

By enriching culture,hydrolysis ring,9 xylanase producing strains were screened from the soil on which corncobs piled for a long time.A high-yield xylanase producing strain was selected,whose characteristics were studied and with which enzymes were produced by fermentation.Results show that xylanase enzyme activity was high;it was 13.68 U/mL when temperature was at 28~30℃,original pH value about 5.0,turning speed 150 r/min,and time 48 h.Xylanase produced was heat1-resisting,its enzyme activity reserving 60.82 after 120 mins undertemperature 60℃.Its optimal temperature and pH value were 53℃ and pH 5.3 respectively.

Key concepts: Xylanase, Strain (injury), Fermentation, Hydrolysis, Enzyme, Yield (engineering), Food science, Chemistry

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