Study on antioxidant activities of extracts from Curculigo orchioides in vitro

Objective To study on antioxidant activities of extracts from Curculigo orchioide in vitro.Methods Curculigo orchioides were treated with 80% ethanol and then the residue were extracted with water.The ethanol extract were extracted respectively using petroleum ether,chloroform,ethyl acetate,n-butanol.At the same time,crude polysaccharide was obtained by ethanol precipitation from water extract.Hydroxyl free radical(·OH),DPPH free radical(DPPH·)and deoxidization Fe3+ reaction system were used to observe in vitro antioxidant effects of the above extracts.Results Compared with the ascorbic acid,the extracts had strong scavenging effect on free radicals,the activities sequence for scavenging ·OH were ethyl acetate extract n-butanol extract polysaccharide ethanol total extract,which corresponding IC50 values were 0.247,0.358,0.796 and 0.871,respectively.The capabilities of scavenging DPPH were ethyl acetate extract n-butanol extract ethanol total extract chloroform extract petroleum ether extract polysaccharide,and the corresponding IC50 values were 0.026,0.055,0.620,0.071,0.122 and 0.275 mg/ml,respectively.Different extracts had strong deoxidization ability of Fe3+,the activities sequence were ethyl acetate extract n-butanol extract ethanol total extract chloroform extract petroleum ether extract polysaccharide.Conclusions Curculigo orchioides have stronger antioxidative activities and will be widely applicated on antiaging.