2003•Grassland and TurfRequires access

The Effect of 2,4-Dichlorophenoxyacetic Acid(2,4-D) and 6-Benzyladenine(6-BA) on Callus Inductionof Bluegrasses

Ding Lu

Abstract

The callus induction of Bluegrasses is the prerequisite and foundation to establish high frequency regeneration system. Callus cultures were obtained from mature seeds of Kentucky bluegrasses(Poa pratensis L.) and Poa sphendylodes. Mature seeds were cultured on Murashige and Skoog medium(MS) supplemented with different 2,4dichlorophenoxyacetic acid(2,4D) and 6Benzyladenine concentrations. The rate of callus induction is strongly different with the cultivar. The effect of 2,4dichlorophenoxyacetic acid (2,4D) combining with 6benzyladenine(6BA) is better than only using 2,4dichlorophenoxyacetic acid.

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Key concepts: 2,4-Dichlorophenoxyacetic acid, Callus, Botany, Cultivar, Chemistry, Biology, Horticulture