Regeneration of green plants from seed-derived callus cultures of Poa

Plants were regenerated from callus derived from entire mature seeds of three Poa pratensis cultivars (Kentucky bluegrass, Fylking and Baron) and one Chinese native species, Poa sphendyLodes L. Kentucky bluegrass showed the highest regenerating capacity of the four cultivars. Addition of low concentration (0.2 mg/l) of (6-benzyladenine) 6-BA to Murashige and Skoog (MS) callus induction medium containing 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) strongly stimulated seeds inducing shoot-forming callus cultures in Kentucky bluegrass. High percentage of shoots was induced in Shenh and Hilde-brandi (SH) medium containing low concentration (0.1 mg/l) of 2,4-D. Roots were obtained in SH medium with 0.1 mg/l NAA (naphthalene-acetic acid).