DISINFECTION EFFICACY AND SUBACUTE TOXICITY OF A SKIN-MUCOUS MEMBRANE DISINFECTION SOLUTION

Objective To study the germicidal efficacy and safety during repeated use of a skin-mucous membrane disinfection solution in order to provide the basis for actual use.Methods Suspension quantitative germicidal test and animal test were used to observe its germicidal efficacy and subacute toxicity.Results The killing logarithm values of Escherichia coli and Staphylococcus aureus in suspension exposed to the disinfection solution containing 200 mg/L available iodine and 12.70 mg/L chlorhexidine acetate for 1 min were over 5.0.The killing logarithm value of Pseudomonas aeruginosa in suspension exposed to the disinfection solution containing 150 mg/L available iodine and 9.53 mg/L chlorhexidine acetate for 1 min was over 5.0.The killing logarithm value of Candida albicans in suspension exposed to the disinfection solution containing 250 mg/L available iodine and 15.90 mg/L chlorhexidine acetate for 1 min was over 4.0.Wiping disinfection with the disinfection solution containing available iodine 4500 mg/L and chlorhexidine acetate 286 mg/L for 1 min killed the natural bacteria on hand and skin surface by a logarithm value of over 1.0 in average.In capacity test of the disinfectant solution in killing E.coli,the minimal eligible concentration was available iodine 100 mg/L and chlorhexidine acetate 6.36 mg/L.In subacute toxicity test,the animal blood routine examination items showed no change,various biochemical parameters were within the normal ranges and no pathological change was found in pathological examination of various organs.Conclusions The skin-mucous membrane disinfection solution consisting of available iodine and chlorhexidine acetate has good killing effect on vegetative forms of bacteria and fungi and does not cause abnormality in subacute toxicity test in rats.