Study on the relationship of FHIT,HPV16E6,HPV16E7 protein expression in cervical carcinoma and in cervical intraepithelial neoplasia

Objective To determine the relationship of fragile histidine triad(FHIT)protein expression and HPV16 E6/E7 oncoprotein expression in cervical carcinoma and in cervical intraepithelial neoplasia.Methods Immunohistochemistry was used to detect the expression of FHIT protein,HPV16 E6 and HPV16 E7 proteins in 15 normal cervices and 25 CINs as well as 61 primary invasive cervical carcinomas.Results In normal cervices,CIN Ⅰ~ Ⅱ,CINⅢ and in invasive cervical cancers,the positive rates of FHIT protein expression were:100%(15/15),71.43%(10/14),36.36%(4/11),and 14.75%(9/61)respectively(P0.05);the positive rates of HPV16E6 protein expression were:0(0/15),7.14%(1/14),36.36%(4/11),and 59.02%(36/61)respectively(P0.05);the positive rates of HPV16E7 protein expression were:20.00%(3/15),42.86%(6/14),63.64%(7/11),and 57.38%(35/61)respectively(P0.05).There was an inverse correlation between the expression of HPV16 E6 protein and FHIT protein(P0.01,r=-0.449).HPV16 E7 had no relationship with FHIT expression(P0.05).Conclusion The abnormal expression of FHIT protein is correlated with the expression of HPV16 E6 protein in cervical cancer.It seems that the detection of FHIT protein and HPV16 E6 protein can be valuable indicators of malignant changes in premalignant cervical lesion.