Study of the Expression of FHIT in Cervical Adenocarcinoma and the Relation to HPV Infection

Objective To study the role of fragile histidine triad (FHIT) gene in occurrence and development of cervical adenocarcinoma,and to analyse the relationship between the expression of FHIT protein and human papilloma virus(HPV) infection.Methods The expressions of FHIT and HPV16/18-E6 proteins were determined by tissue microarray combined with immunohistochemistry in 86 cases of cervical adenocarcinoma and 24 cases of cervical chronic inflammation.Results The positive rate of FHIT in cervical adenocarcinoma was 46.5%,which significantly lower than that in cervical chronic inflammation 100.0%(P(0.01)).The positive rate of HPV16/18-E6 protein in cervical adenocarcinoma was 65.1%,which significantly higher than that in cervical chronic inflammation 4.2%(P0.01).In cervical adenocarcinoma the loss of FHIT expression was significantly related to pathological grades and histological types.The positive rates of FHIT in G_2 and G_3 are 39.4% and 22.7%,which significantly lower than in G_1(P0.05).The level of FHIT positive expression in clear-cell adenocarcinoma is 22.2%,which significantly lower than in other types(P0.05).The expression of HPV16/18-E6 protein was correlated neither with pathological grades nor histological types(P(0.05)).Conclusion Carcinogenesis and development of cervical adenocarcinoma are closely related to the loss of FHIT expression and HPV infection.