2004•Chinese journal of integrated traditional and Western medicineRequires access
Effects of Ganlike on the mRNA Expression of iNOS in Rats with Experimental Hepatic Fibrosis
Xia Liang
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Abstract
Objective:To investigate the mRNA expression of the inducible nitric oxide synthase and the effects of Ganlike in rats with experimental liver fibrosis induced by carbon tetrachloride. Methods:SD rats′model of fibrosis was induced by injecting CCl 4. After model being built, they were protected with Ganlike and Colchicine, and normal saline were taken as the control. The expression of iNOS mRNA in all liver tissues was detected by in situ hybridization method. The severity of inflammation and fibrosis in all liver tissuses were measured by semiquantitative scoring. Results: the expression of iNOS mRNA in the hepatic fibrosis were significantly higher than the control group(P0.01), after the treatment with Ganlike, The severity of inflammation and fibrosis were obviously improved, and the expression of iNOS mRNA in hepatic fibrosis was downregulated. Conclusion:Ganlike can downregulate the expression of iNOS mRNA in hepatic fibrosis, which maybe a possible mechanism of its anti-hepatic fibrosis.