2005•Unpublished venueRequires access
EFFCT OF GANLIKE ON ET-1 mRNA and iNOS mRNA EXPRESSION IN LIVER TISSUE OF RATS WITH HEPATIC FIBROSIS
Xia Liang
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Abstract
Objective:To observe the effect of ganlike on ET-1mRNA and iNOS mRNA expression in liver tissue of rats with hepatic fibrosis and discuss the mechanism.Mthods:the model of rats′ hepatic fibrosis were induced by subcutaneous injection of CCl_4, then the rats in the treatment groups were given the different dosages of ganlike by stomach-perfusion,the rats in the groups of the model and the normal were given saline,the mRNA expression of ET-1 and iNOS in all liver tissues was detected by in situ hybridization method.Results:the mRNA expression of ET-1 and iNOS in the hepatic fibrosis were significantly higher than that of the normal group(P0.01),after treated with ganlike,the severity of inflammation and fibrosis were obviously improved,and the expression of iNOS mRNA in hepatic fibrosis was downregulated.the expression of ET-1 mRNA showed no significance.Conclusion:ET-1 and iNOS participate in the occurrence and development of hepatic fibrosis,ganlike may downregulate the mRNA expression of iNOS in the liver tissues of hepatic fibrosis,which may be a possible mechanism of anti-hepatic fibrosis.