Study on the Differentially Expressed Genes in Ameloblastoma Using cDNA Microarry.

Objective:To screen the differentially expressed genes between ameloblastoma and normal oral mucosa using cDNA microarray.Methods:The PCR products of 700 genes were spotted onto a kind of chemical-material-coated-glass slide.The total RNAs were isolated from ameloblastoma and normal oral mucosa,respectively,and were purified to mRNAs by oligotex.Both the mRNAs from two kinds of tissues were reversely transcribed to cDNAs with the incorporation of fluorescent-labelled dUTP to prepare the hybridizaton probes.The mixed probes were hybridized to cDNA microarray. Tumor-related genes were screened through the analysis of fluorescent intensity.Results:Among 700 target genes,32 differentially expressed genes were found between ameloblastoma and normal oral mucosa tissues .Up- and down-regulated genes were 5 and 27 respectively.Conclusions:cDNA microarray technique was effective in screening the differentially expressed genes between two different kinds of tissues.Further analysis of those obtained genes will be helpful to anderstard the molecular mechanism of ameloblastoma.