Screening differentially expressed genes of gastric adenocarcinoma by cDNA microarray

OBJECTIVE:To screen differentially expressed genes between gastric adenocarcinoma and normal mucosa using cDNA microarray.METHODS:The PCR products of 8192 human genes were spotted onto a chemical material coated glass plates in array.DNAs were fixed on the glass plate after series of treatments.The total RNAs were isolated from the tissues,and were purified to mRNAs by Oligotex.Both mRNAs from the gastric adenocarcinoma and normal gastric mucosa were reversely transcribed to the cDNAs with the incorporation of fluorescent dUTP to prepare the hybidization probes.The mixed probes were hybridized to the cDNA microarray.After high stringent washing,the cDNA microarray was scanned for fluorescent signals and the differences between the two tissuses were compared.RESULTS:Among the 8 192 target genes,the expression level of 327 genes was different between gastric adenocarcinoma and normal mucosa,179 were down regulated and 148 up regulated.CONCLUSIONS:Many genes take part in the carcinogenesis of gastric adenocarcinoma.cDNA microarray technique is effective in screening the differentially expressed genes between gastric adenocarcinoma and normal mucosa.