2006•Xibei Linxueyuan xuebaoRequires access
A Study on Tissue Culture of Fine Variety "Shiyou 1" of Hippophae rhamnoides
Zhou Song-kun, Zongqin Zhang
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Abstract
The hydroponics stem apexes and leaves of Shiyou 1(Hippophae rhamnoides) were used as explants to induce adventitious buds and callus.The results showed that adventitious buds and callus could be obtained from stem apexes on 1/2 MS + 6-BA 1.0 mg·L~(-1) + IAA 0.5 mg·L~(-1),and cluster shoots were got by subculture.Axillary buds could be induced on 1/2 MS+ 6-BA 2.5 mg· L~(-1)+ IAA 0.2 mg·L~(-1) from juvenile stems,but the propagation rate was low.On 1/2 MS + 6-BA 0.5 mg·L~(-1) + KT 0.2 mg·L~(-1)+ NAA 0.02 mg·L~(-1),an average of 9.1 adventitious buds per explant were induced effectively and easily from hydroponics leaves after thirty days,the differentiation rate was up to 94.1%.On 1/2 B_5 + 6-BA 0.3 mg·L~(-1) + IBA 0.4 mg·L~(-1),roots were induced with a high inducing rate of 87.5% on the subcultured plantlets.