Extraction and initial identification of active principle oxymatrine from Xinjiang Sophora alopecuroides

Objective To study the isolation technology and initial identification of oxymatrine from Xinjiang Sophora alopecuroides.Methods The crude oxymatrine product was prepared from the seed of Sophora alopecuroides by ethanol reflux extraction and by ethanol ultrasonic extraction methods,respectively.Target product oxymatrine was purified by using macroporous absorbent resin,and the preparation technology was optimized.Results The optimal condition of ethanol reflux extraction: solvent 60% of ethanol,reflux for 6 h at 60 ℃,the solid /liquid ratio 1:15;the condition for ultrasonic extraction: solvent 60% of ethanol,.extraction frequency 28 MHz,extraction time 20 min at 60 ℃,the solid/liquid ratio 1:15.The prepared crude products were purified by LSA-21 macroporous resin(pH =10) within gradient eluant of ethanol(60%,45% and 30%) in which,the efficiency of 30% ethanol is the better.Conclusion The ethanol reflux extraction and the ultrasonic extraction were feasible methods for preparing total alkaloids from Sophora alopecuroides,in which,the ultrasonic extraction has advantages such as energy saving,rapid,less environment polluting,but with less extraction rate than those of ethanol-reflux extraction.The use of LSA—21 macroporous resin is a suitable method for purifying target product oxymatrine with lower economic cost.Furthermore,this work provides a simple technology for rapid preparation of pure oxymatrine in laboratory.