OPTIMIZATION OF TOTAL PHENOL WATER EXTRACTION FROM CHAENOMELES SINESIS VIA RESPONSE SURFACE METHODOLOGY
Zheng Hai-yan
Abstract
Zheng Hai-yan
Abstract
On the basis of the previous results, the method of Response Surface Method with extraction temperature, solid-liquid ratio and extraction time was adopted according to the central composite experimental design principles of Design Expert.Then the effects of each factor on total phenol water extraction rate were determined by means of regression analysis.Response surface and contour were finally graphed with the total phenol extraction rate as the response value.The results showed that the optimum conditions of total phenol water extraction from chaenomeles sinesis were as follows:the extraction temperature of 86.5 ℃,solid-liquid ratio of 1:23.7 g/mL and extraction time of 1.5 h.The order of primary effects was:extraction temperature solid-liquid ratioextraction time.The founded model could explain 95.91 % changes of the response value,and the correlation coefficient of predictive value(92.98 mg/g) and measured value(94.46 mg/g) was 0.978 5,so the model could be used in practice credibly.
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On the basis of the previous results, the method of Response Surface Method with extraction temperature, solid-liquid ratio and extraction time was adopted according to the central composite experimental design principles of Design Expert.Then the effects of each factor on total phenol water extraction rate were determined by means of regression analysis.Response surface and contour were finally graphed with the total phenol extraction rate as the response value.The results showed that the optimum conditions of total phenol water extraction from chaenomeles sinesis were as follows:the extraction temperature of 86.5 ℃,solid-liquid ratio of 1:23.7 g/mL and extraction time of 1.5 h.The order of primary effects was:extraction temperature solid-liquid ratioextraction time.The founded model could explain 95.91 % changes of the response value,and the correlation coefficient of predictive value(92.98 mg/g) and measured value(94.46 mg/g) was 0.978 5,so the model could be used in practice credibly.
Key concepts: Response surface methodology, Extraction (chemistry), Chromatography, Chemistry, Phenol, Organic chemistry