Observation on effect of avian influenza (H5N1) vaccine with immunization animals

Objective The vaccines produced according to the three techniques with the virus seeds R1203 of H5N1 avian influenza were used to immunize animals and to observe the effect.Methods Rats and rabbits were immunized with the different dose of the three vaccines of avian influenza(H5N1) by an intramuscular injection twice(interval 14 days).Blood was collected intravenously in the day of 14 and 28.The hemagglutination inhibition antibody and neutralization antibody occurred in the serum of the animals after the first and second inoculation.Results The antibody reaction in the rats was more sensitive than the rabbits.The rats and rabbits both could produce hemagglutination inhibition antibody in 14th day with the titer of the antibody reaching at 1:40 while the titer of the neutralization antibody was very low and hardly detected.The second inoculation were significantly higher than the first inoculation.The antibody reaction after inoculation of the split-2 vaccine in the rats and rabbits were significantly stronger than that inoculated with another two vaccines.The quantum-effect reaction was distinct in the rabbits.Conclusion The production technique of split-2 vaccine was better than the other two.The neutralization antibody can accurately reflect the quality of vaccines.The efficiency test should be added in vaccines research and production with the method of immune rabbits.It is suggested to immunize rabbits twice with 12 μg HA with the titer of the sera-neutralization antibody reaching at 1:40 or over in animal test before clinical trial.