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2013•Chinese Journal of Tropical AgricultureRequires access

Determination of Gibberellin Residue in Banana by High Performance Liquid Chromatography

Xueqiang Zhang

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Abstract

A method for the determination of gibberellin residue in banana sample was established with high performance liquid chromatogram coupled ultraviolet detector(HPLC-UVD).Residues of gibberellin were extracted from sample with methanol.After been cleaned up,the final extract was injected into HPLC-UVD.The detection limit was 0.1 mg/kg,Precision and recovery tests were made at the spike level of 0.50~10.0 mg/kg,and the results were as follows: recoveries in the range of 86% ~105%,relative standard deviation 2.0%~4.8%.

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What this paper is about

A method for the determination of gibberellin residue in banana sample was established with high performance liquid chromatogram coupled ultraviolet detector(HPLC-UVD).Residues of gibberellin were extracted from sample with methanol.After been cleaned up,the final extract was injected into HPLC-UVD.The detection limit was 0.1 mg/kg,Precision and recovery tests were made at the spike level of 0.50~10.0 mg/kg,and the results were as follows: recoveries in the range of 86% ~105%,relative standard deviation 2.0%~4.8%.

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Available abstract

A method for the determination of gibberellin residue in banana sample was established with high performance liquid chromatogram coupled ultraviolet detector(HPLC-UVD).Residues of gibberellin were extracted from sample with methanol.After been cleaned up,the final extract was injected into HPLC-UVD.The detection limit was 0.1 mg/kg,Precision and recovery tests were made at the spike level of 0.50~10.0 mg/kg,and the results were as follows: recoveries in the range of 86% ~105%,relative standard deviation 2.0%~4.8%.

Key concepts: Gibberellin, Chromatography, Residue (chemistry), High-performance liquid chromatography, Detection limit, Chemistry, Relative standard deviation, Methanol

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