Effect of Fangjifulingtang on Proliferation and PAI-1 in Glomerular Mesangial Cells

Objective To investigate the effect of Fangjifulingtang on preventing proliferation of glomerular mesangial cells and the expression of PAI-1, so as to provide experimental evidence for Fangjifulingtang to cure chronic kidney disease. Method Glomerular mesangial cells were cultured and TNF-α was used as a stimulation to make the cells proliferate. Then Fangjifulingtang was incubated together at different doses. Cell vitality were evaluated and qualified by MTT. Cell period were analysed by Flow CytoMeter (FCM). Secretion of PAI-1 expression was measured by ELISA. Results The proliferation of Glomerular mesangial cells was inhibited (P0.05). The DNA diagram of Flow CytoMeter showed that the cell quantity in stage ‘S’ was reduced (P 0.05) and the expression of PAI-1 was significantly inhibited (P 0.05). Conclusion Fangjifulingtang was able to inhibit proliferation of glomerular mesangial cells and influence the expression of PAI-1, so as to regulate the synthesis and degradation of extracellular matrix.