Effects of TGF-β1 on expression of PAI-1 in rat mesangial cells

Objective To study the effects of transforming growth factorβ1(TGF-β1) on mesangial cells growth and expression of plasminogen activator inhibitor-1(PAI-1) in cultured rat mesangial cells stimulated by different levels of human recombinant TGF-β1.Methods The proliferation of mesangial cells was determined with MTT assay and FACS analysis.The levels of PAI-1 protein and mRNA were analyzed with Western blotting analysis and RT-PCR respectively.Results Compared with control group,0.1 and 0.5 μg·L~(1) TGF-β1 stimulated mesangial cells proliferation,the proliferation rate of mesangial cell was(146.6±10.2)% and(134.6±14.5)% respectively,and the expressions of PAI-1 mRNA and protein also increased(P0.05).The proliferation rate was(75.2±13.2%) and(67.5±11.1)% in the group treated with 5 and 25 μg·L~(-1) TGF-β1,the expressions of PAI-1 mRNA and protein were constant.Conclusion Low level of TGF-β1 can trigger the proliferation of rat mesangial cells,induce the expression of PAI-1,and high level of TGFβ1 can inhibit the proliferation of mesangial cells.