In vitro Tissue Culture and Rapid Propagation of Sour Cherry

The technology of tissue culture and rapidly propagation of sour cherry was studied using new shootings and stem sections with axillary bud as explants.There were 2 kinds of basal cultural medium(MS and F(14)),2 kinds of hormone with different concentration [6-benzyladenine(6-BA) and 3-indole butyric acid(IBA)].The results showed that the buds grown better in MS than that in F(14) medium.MS medium with 0.5 mg·L(-1) 6-BA and 0.2 mg·L(-1) IBA was the most suitable for the propagation of sour cherry and the propagation rate reached 4.46 times.The effective number of new shoot was up to 83.6%.On the other hand,the good medium to induce root was 1/2 MS+0.5 mg·L(-1) IBA with 71.4% of rooting rate and(2.1) cm of average root length.There was higher proportion of viability planted test-tube seedlings in the substratum(vermiculite),the survival rate was up to 80%.