Effects of Genistein on Proliferation, Apoptosis and Cisplatin Sensitivity of Drug-resistant Human Ovarian Carcinoma Cell SKOV-3

Objective To study the effects of genistein on proliferation, apoptosis and cisplatin sensitivity of drug-resistant ovarian carcinoma cell skov-3. Methods MTT assay was used to analyze growth inhibitory effect of genistein alone and with cisplatin on SKOV-3 cell. Cell cycle arrest and apoptosis was meas-ured by flow cytometry (FCM). Results Genistein exerted inhibitory effect on SKOV-3 cell in a concen-tration-dependent way and significantly enhanced its sensitivity to cisplatin(P 0.05). Generally, cisplatin ( 0.625~5μg/ml) combined with genistein ( 2.5~10μg/ml) resulted in synergistic effect. Both 5μg/ml and 10μg/ml genistein arrested SKOV-3 cell in the G2/M phase and induced apoptosis while 10μg/ml genistein caused additional disturbance on S phase process. Significant synergistic effect between genistein and cisplatin was found in inducing cell cycle arrest and apoptosis. Conclusion Genistein can inhibit the growth of drug-resistant human ovarian carcinoma cell SKOV-3 and enhance its sensitivity to cisplatin significantly.