Effect of Genistein on increasing apoptosis of drug-resistant ovarian cancer cell SKOV-3 induced by cisplatin.

Objective:To investigate the mechanism of genistein combined with cisplatin-induced apoptosis in drug-resistant ovar-ian cancer cell SKOV-3.Methods:The morphological alterations of SKOV-3 cells were demonstrated by microscope.The growth in-hibitory effect of different drugs on SKOV-3 cells was detected by MTT assay.The apoptosis rate was determined by flow cytometry.The level of ROS was measured by both flow cytometry and Fluorescence Microscope.Results:After co-treatment of 10ug/ml genistein and 2.5ug/ml cisplatin for 24h,the level of ROS increased obviously,and the apoptotic rate was also significantly raised,as compared with cisplatin alone(P0.05).Pretreatment with antioxidant NAC,ROS was effectively inhibited,cell viability was increased and the apoptotic rate was decrased.Conclusion:Genistein potentiated cisplatin-induced apoptosis in drug-resistant ovarian cancer cell SKOV-3 is related to the increased ROS.It may be one of the mechanisms of apoptosis induced by the combination in SKOV-3 cells.