Synergistic Effect of Genistein and Cisplatin on the Proliferation of Hepatoma Cell Line in Vitro

Objective To explore the synergistic effect of genistein and cisplatin on the proliferation of hepatoma cell line HCCLM3.Methods The inverted phase contrast microscope was used to observe the influence of combined cisplatin and genistein on the morphosis of HCCLM3 cells.MTT method was used to detect the effect of cisplatin combined with genistein on the proliferation of hepatoma cell line (HCCLM3 cells),and the median-effect principle and formula was used to computate the synergetic effect.The flow cytometry was used to detect the influence of the combined two drugs on cell apoptosis and cell cycle.Results By the inverted micro-scope,lower cell density was observed in genistein combined with cisplatin group than the single drug group. Cells in the combined drug group inter-separated,and the lucency of cytoplasm decreased.In genistein combined with cisplatin group,cell survival rate decreased more significantly than in single drug group.With the increase of drug concentration,the combination of genistein and cisplatin performed coordinate repression on the proliferation of HCCLM3 cells.Genistein combined with cisplatin had more significant effect in inducing apoptosis compared to the single drug,mainly by blocking the G0/G1 stage.Conclusions With the increase of concentration,genis-tein combined with cisplatin has synergistic repression on the proliferation of HCCLM3 cells in vitro. The mechanism may partly lies in that genistein combined with cisplatin possesses robuster capacity to induce apoptosis in cancer cells than they are single used.