Effects of Xiangfu Siwu Decoction on Nerve Endocrine Immune System in Female Rats with Qi Stagnation and Blood Stasis Syndrome

Objective: To study the effects of Xiangfu Siwu decoction on the blood,nerve endocrine immune( NEI) system in female rats with Qi stagnation and blood stasis syndrome. Method: The female SD rats model with Qi stagnation and blood stasis syndrome were prepared by giving adrenaline( Adr) and chronic unpredicted stress, while the medicine groups were treated with Xiangfu Siwu decoction. ELISA and immunohistochemistry assay were employed to detecte contents of interlerkin-6( IL-6),leukotrienes 4( LTB 4), estradiol( E 2),progesterone( P),prostaglandin E 2( PGE 2),nitric oxide( NO) in the serum and β-endorphin in hypothalamus before and after the treatment. PLS-DA( Patrial least squares discriminant analysis) method was used to analyze the above factors. Result: Compared with control group,the model group had significantly higher levels on hemorheology and significantly lower levels on thrombin time( TT) and activated partial thromboplastin time( APTT),while the model group had no obvious changes on prothrombin time( PT) and fibrinogen; the model group had significantly higher levels on the content of IL-6,P in the serum,and significantly lower levels on the contents of E 2,PGE 2,NO in the serum,while the model group had no obvious changes on the content of LTB 4. Xiangfu Siwu decoction had a significant effect on improving the model with Qi stagnation and blood stasis syndrome. Compared with control group,the medicine groups had no significant difference. Through a multi-index analysis with PLS-DA,the most important X variables were E 2,PGE 2,P,TT,horizontal movement,area,vertical movement,integral optical density( IOD),30 s- 1whole blood riscosity,residence time,plasma viscosity. Conclusion: The therapeutic effects of Xiangfu Siwu decoction on many diseasesmay be mediated by modulating multi-level of blood,nerve,immune,endocrine system and keeping internal environment homeostasis.