Study on compare Taohong Siwu Decoction,Taoren-Honghua pair and Siwu Decoction for primary dysmenorrhea

Objective: Compare Taohong Siwu Decoction,Taoren-Honghua herb pair and Siwu Decoction for primary dysmenorrhea of gynecologyblood stasis syndrome and clarify formulation rules.Methods: The rats were ice water soaked as stimulation for 5 min between 2 injections of epinephrine hydrochlorides to build rats' acute blood stasis mode and administrated 5 times of clinical dosage.Determine hemorheology and plasma coagulation and observe uterine and ovarian histological changes by HE staining.The rats were injected acetylphenylhydrazine and cyclophosphamidum to build rats' blood-deficient mode and administrated clinical dosage.Determine changes of peripheral hemogram and observe morphological change of spleen,thymus gland and liver.The rats were injected estrogens and oxytocin to build mice's primary dysmenorrhea mode and administrated clinical dosage..Observe writhing response in 30 min.Levels of Ca 2 + and nitric oxide(NO) in primary dysmenorrheal model mice uterus were also been detected.Results: In rats' acute blood stasis mode,THSW could significantly decrease whole blood viscosity at low shear rate and prolong thrombin time.TH could decrease whole blood viscosity,plasma viscosity,erythrocyte sedimentation rate blood and packed cell volume and prolong thrombin time,prothrombin time and thromboplastin time and decrease fibrinogen content.However,SW had no effects on these indexes.SW,THSW and TH all had a good effect on uterine and ovarian histological changes.In rats' blood-deficient mode,SW and THSW could significantly increase RBC,increase MCV and liver index.SW also was in favor of spleen histological changes.TH could significantly increase WBC and PLT.In primary dysmenorrhea mode,THSW could decrease writhing response which was relation to decrease level of Ca 2 + and increase level of NO.TH could also decrease writhing response but the mechanism was unknown.Conclusions: Compare to SW,THSW not only had function of SW but also had stronger effects on promoting blood circulation through influencing on hemorheology,plasma coagulation because of adding TH herb pair in THSW.